Dr. Pusp Raj Joshi is a Nepalese botanist, plant biotechnology researcher, and academic professional specializing in orchid conservation, plant tissue culture, phytochemistry, and the evaluation of biologically active compounds from medicinal plants. He is affiliated with the Annapurna Research Center, Kathmandu, where he has been serving as a Senior Research Officer since March 2016. He has extensive experience in plant tissue culture, in vitro propagation, molecular laboratory techniques, cell culture, and the isolation and characterization of bioactive compounds. Dr. Joshi obtained his Bachelor of Science (B.Sc.) in Botany in 2013 and Master of Science (M.Sc.) in Botany in 2017 from Tribhuvan University, Nepal. His doctoral research in Botany at the Central Department of Botany, Tribhuvan University, focuses on evaluating the cytotoxic effects of bioactive compounds derived from in vitro-raised protocorms of Dendrobium transparens Wall. ex Lindl. and Dendrobium heterocarpum Wall. ex Lindl. on human cancer cell lines. His research interests include the conservation and micropropagation of threatened and commercially important orchid species, plant biotechnology, phytochemical profiling, antioxidant and anticancer activities of plant-derived compounds, and the development of plant-based biomedical applications. He has contributed to several research projects supported by national and international organizations, including the University Grants Commission (UGC), Taiwan Orchid Growers Association, KOICA/IRDP, and the Nepal–Denmark cooperation programme (UNNATI). His research contributions also include work on the in vitro propagation of Gastrochilus calceolaris, a critically endangered orchid of Nepal, and the development of disease-free cardamom through micropropagation. In addition to his research responsibilities, Dr. Joshi has been involved in teaching Botany at various academic institutions since 2017. He has supervised and supported undergraduate and postgraduate research projects, contributed to scientific publications, and participated in national and international scientific conferences and training programmes. His research has received recognition through the Best Poster Award at the First PhD Festival in October 2023 and the Best Paper Award at the World Orchid Conference 2021 for research on in vitro seed germination of Gastrochilus calceolaris. Through his combined experience in research, teaching, and plant biotechnology, Dr. Joshi contributes to orchid conservation, sustainable utilization of plant resources, and the advancement of plant-based biomedical research in Nepal.